Animal Cell Culture (Hardback, Ratan Kumar Choudhary, Manu M & Yashpal Singh MaliK)

Animal cell culture is a common laboratory technique for maintaining live cells separated from their original tissue (primary cells) or immortal cells (cell lines). The technique of cell culture was discovered in the 19th century after the discovery of the microscope.

Presently, the culture of cells is one of the most important tools of life sciences research and is being used by investigators to understand advanced understanding of cell growth and differentiation and mechanisms of normal/ abnormal functioning of various cells. In addition to that, cell culture is employed in the generation of vaccines, the production of pharmaceutical proteins, and the safety testing of drugs.

Content

1 Layout, Safety Protocols and Guidelines in Cell Culture Facilities

2 Basic Equipment of Animal Cell Culture Laboratories

3 Washing, Packing, and Sterilization of Glass and Plastic Wares

4 Preparation of Cell Culture Media and Reagents

5 Isolation of Primary Culture of Chicken Embryo Fibroblast

6 Isolation of Primary Culture of Canine Keratinocytes

7 Isolation of Primary Culture of Canine Fibroblast

8 Culture and Sub-culturing of Continuous Cell Lines

9 Trypan Blue Dye Exclusion Cell Viability Assay

10 Isolation and Culture Techniques for Lymphocytes

11 Cryopreservation Methods of Primary Cultures and Cell Lines

12 Evaluating Cytopathic Effects of Viruses on Mammalian Cells

13 Virus Isolation in Cell Culture System

14 One-Step Growth Curve of a Virus

15 Production of Virus in Cell Culture

16 Quantitative Analysis of Virus Titers through In-Vitro Assays

17 Hemadsorption (HA) Assay for Virus Titration

18 Plaque Assay for Virus Detection and Quantification

19 Virus Neutralization Test

20 Techniques for Establishing and Maintaining Cell Suspension Cultures

21 Development of Cell lines Through Virus-Mediated Techniques

22 Cell Viability and Cell Proliferation Assay